Fermentas（MBI ）自1975年成立至目前成功与Thermo合并以来一直为世界分子生物学科研领域提供和方便的产品。作为其产品之一，在中国用户中的反映有口皆卑。试剂盒提供oligo(dT)18和随机多态引物，同样适合原核和真核生物的反转录。合成过程中，核酸酶抑制剂可减缓mRNA降解速度，以便能获得全长的cDNA*链，zui长可以反转录9kb的片段。使用快捷、稳定，在众多品牌同类产品中其销量屡见新高。

在M-MuLV逆转录酶的作用下，将mRNA转录为cDNA的*链。合成过程中，核酸酶抑制剂的加入可减缓mRNA降解速度，以便能获得全长的cDNA*链。

Description

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation.

The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of the poly(A) tail, therefore, they can be used for transcription of the 5’-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs). Gene-specific primers may also be used with the kit.

Highlights

• Full-length first strand cDNA up to 13 kb
• Optimum reaction temperature 42°C
• Complete kit – all the components for the RT reaction are included

Applications

• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

Includes

The RevertAid First Strand cDNA Synthesis Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Oligo(dT)18 Primer, Random Hexamer Primer, Control GAPDH RNA, 10 μM Forward GAPDH Primer, 10 μM Reverse GAPDH Primer, and nuclease-free water.

The RevertAid RT Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Random Hexamer Primer, and nuclease-free water.

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